Musical Theatre Karlín, Neo-Renaissance theatre in Karlín district, Prague, Czech Republic.

Musical Theatre Karlín is a neo-Renaissance theatre building on the edge of Karlín near Florenc, designed by architect Otto Ehlen and built by František Kindl. Its stage was engineered for heavy loads, including trained animals such as elephants. The auditorium seats 885 and the interior features stucco work, chandeliers and murals by Max Švabinský.

Eduard Tichy, a Prague grain merchant, bought land near Florence and had a neo-Renaissance theatre built by architect Otto Ehlen, opening on 27 August 1881 as Théâtre Variété with a Belgian circus. The stage was engineered for heavy loads, including trained elephants. The family sold it in 1920; it served as a variety hall until 1939.

The theatre regularly presents Czech and international musicals on its stage. Productions range from classic operettas to contemporary shows, attracting audiences from across the city.

The theatre stands near the Florenc metro station and Prague's main bus terminal, on the edge of Karlín. The building is wheelchair accessible. The auditorium seats 885 people, and the stage is the largest in Prague.

The stage was engineered for heavy loads because trained animals, including elephants, were meant to perform; the Belgian Royal Circus opened the house on 27 August 1881 with four horses and horse-drawn carriages. Charlie Chaplin and Stan Laurel are said to have appeared here early in their careers.