Church of Saint Bartholomew, Gothic church in Kolín I, Czech Republic.

The Church of Saint Bartholomew is a Gothic building of pale stone with a steep red-tiled roof. Its early Gothic western part has two octagonal towers 66 metres high, while on the north side stands a separate late Gothic bell tower with a gallery once used as the watchman's flat. Behind the western mass rises Petr Parléř's tall 14th-century chancel, ringed by a crown of six chapels between buttresses.

The early Gothic church was probably founded shortly after Kolín became a royal town in the mid-13th century, likely by King Přemysl Otakar II, and was completed before 1300 with two octagonal west towers. After a fire in 1349, Charles IV commissioned Petr Parléř in 1360 to build a new high chancel; the restored church was consecrated in 1378.

The six chapels around the chancel were once maintained by the town guilds: the Miller's, Maltster's, Butcher's, Šperling, St John's and St Wenceslas chapels. In the Maltster's chapel lies Jan II Žehrovský of Kolovrat, a military captain of the town who took part in the peace mission sent by King George of Poděbrady.

The church stands on the highest point of Kolín's historic centre, with the separate late-Gothic bell tower just to the left of the west front. The main entrance today is the northern portal, nearer the western end of the north facade; the old west portal has been out of use since the 13th century. The west facade and its two 66-metre octagonal towers are best seen from the square below.

The separate bell tower was built in 1504 after bell-ringing disturbed the statics of the north tower; the bells were moved there, and until 1756 they hung with their hearts upward. In 1728 the clock from the north tower was transferred to its wall.