Augustinian monastery in Třeboň, Religious monument in Třeboň, Czech Republic.

The Augustinian monastery in Třeboň consists of the Gothic Church of St Giles and the Virgin Mary, built after 1370, with the Chapel of St Vincent, and a cloister with polychrome ribbed vaults and early 15th-century frescoes. The monastic buildings were rebuilt in late Baroque style in 1750.

The monastery was founded in 1367 by four sons of Petr I of Rožmberk, whom Archbishop Jan Očko of Vlašim authorised on 12 May 1367 to convert the parish church of St Giles into a house of Augustinian canons. The Rožmberks endowed the first eight canons, three sent from Roudnice nad Labem. In 1389 Pope Urban VI raised the provost to abbot. Closed in 1566 under Vilém of Rožmberk, it was restored in 1631 by Ferdinand II and dissolved in 1785 under Joseph II's reforms.

The monastery's church of St Giles and the Virgin Mary Queen, with its Gothic two-nave and the chapel of St Vincent, is shown by the churchwarden on tours every day except Sunday at 3 pm, together with the Gothic cloister and its 15th-century wall paintings and the paradise garden with its well.

Tours of the Church of St Giles and the Queen of Heaven and part of the monastery, including the Gothic cloister with 15th-century wall paintings and the paradise garden with its well, are led by the churchwarden daily except Sundays at 3 p.m. The grounds are accessible for prams and partly for wheelchairs.

The monastery was closed in 1566 by Vilém of Rožmberk after discipline collapsed; its archives and library passed to him. Reopened by Ferdinand II in 1631, it was administered by the abbot of Klosterneuburg until 1663, and canons from Austria first lodged in Třeboň Castle.