Holy Trinity Church, Gothic church building in Královo Pole, Czech Republic.

Holy Trinity Church is a building with Gothic architecture in Královo Pole that forms part of the Charterhouse of Brno. It displays typical features of this style such as pointed arches and ribbed vaults inside.

Margrave John Henry of Moravia and his sons Jošt, Jan Soběslav and Prokop founded the church and charterhouse by a deed of 13 August 1375, three months before his death. The hall church with paired oratories was finished by 1387; Prokop was buried in the choir of the fathers. After a fire and Thirty Years' War damage, it was rebuilt in late Baroque style and reconsecrated on 31 August 1765 by Bishop Maximilian of Hamilton.

The monastery was dissolved by Joseph II in 1782 and the church became a parish church; a baptismal font and a west gallery with an organ were added. The monastery buildings now house the Faculty of Information Technology of Brno University of Technology.

The church stands on Božetěchova Street in Brno-Královo Pole, within the former Carthusian monastery whose buildings now house the Faculty of Information Technology of Brno University of Technology. The whole monastery complex is protected as a cultural monument of the Czech Republic. The church is oriented, with the presbytery to the east and the concave west façade facing the street.

In 1975, while repairing the choir floor, an unknown tomb was found and identified as the burial place of Margrave Prokop of Luxembourg, son of the founder Jan Jindřich. His remains, examined by anthropologist Emanuel Vlček, were placed in a new burial chamber in the Angel Chapel on 21 December 1988.