Church of the Annunciation of the Virgin Mary, Gothic church in Pardubice-Staré Město, Czech Republic.

The Church of the Annunciation of the Virgin Mary in Klášterní Street has a rectangular nave with a lower three-sided presbytery and a sacristy on the south side. The entrance facade carries a triangular gable with pointed arches, and the nave's three bays of cross vaults bear Pernštejn coats of arms on their round bosses.

Founded around 1359 by Archbishop Arnošt of Pardubice and his brothers as the town's oldest church, it was rebuilt in late Gothic style after a 1507 fire. Swedish troops under General Torstenson burned it in 1645, and the adjoining monastery was abolished in 1786. Reopened in 1800 and consecrated in 1815, it was restored in 1904–1905 under Boža Dvořák.

The church is named for the Annunciation of the Virgin Mary and was founded around 1359 by Archbishop Arnošt of Pardubice and his brothers. Services and concerts are held here today, and the organ is still used during Sunday children's masses.

The church stands in Klášterní Street, linking Pernštýnské Square with Komenského Square. The crypt has its own separate entrance from outside. Services and concerts are held here, and most of the furnishings are currently under restoration.

The church is a cultural monument of the Czech Republic, protected since 1958, not a national monument. East of it stood a four-storey cylindrical bell tower built by the Pernštejn master Petr around 1535 and finished in 1541, with an octagonal bell storey and a Renaissance helm; it was meant to be the town's main tower but lost that role to the Green Gate in 1542 and vanished during the Thirty Years' War.