Jardim Botânico de Brasília, Botanical garden in Brasilia, Brazil.

The Jardim Botânico de Brasília spreads across a large area with walking trails that pass through collections of native and introduced plants from different regions. The grounds are organized into distinct sections that represent various plant communities and ecosystems.

Lúcio Costa's 1957 Plano Piloto report already mentioned a botanical garden, but the site moved after the Bananal and Torto streams proved unsuitable. In 1976 a commission chose the 526-hectare Estação Florestal Cabeça de Veado, confirmed in 1982. The garden opened on 8 March 1985 with governor José Ornelas de Souza Filho and botanist Graziela Maciel Barroso present, and became autonomous from the Fundação Zoobotânica in February 1993.

The Alameda das Nações e dos Estados, conceived by Georges Lodygenski, was planned from the garden's start but only began in 2017, when Israel installed the first foreign plot, the Praça de Israel and Jardim Bíblico. A Polish garden represents Poland; Serbia and Oman have shown interest. The Biblioteca da Natureza lends children's books, textbooks, comics and environmental toys.

The garden is open Tuesday to Sunday, 9 am to 5 pm. Access is from the settlement of Jardim Botânico (SMDB Conj. 12, reached from QI 23 Lago Sul); the park centre is reachable by car on the paved Mother Trail. The Trilha Ecológica is 3.5 km long and passes through several Cerrado types.

The garden was the first in the world to keep its plant collections in situ, in their original Cerrado environment, rather than moving specimens into beds. Its 4,429.63-hectare Estação Ecológica holds preserved Cerrado, and about 25% of the springs feeding the region, including Lago Paranoá, rise within the garden's area.