Karoo National Park, National park and nature reserve in Central Karoo District Municipality, South Africa.

Karoo National Park is a protected nature reserve in the semi-arid landscape of Western Cape, featuring vast plains interrupted by mountain ridges. The terrain supports wildlife populations adapted to dry conditions and displays varied vegetation patterns across its expanse.

In the late 1950s farmer and bird enthusiast William Quinton campaigned for a reserve near Beaufort West. In the 1970s the national parks board proposed a park to protect the Nama Karoo biome, and the town donated 7,209 ha as its core. The park was proclaimed in 1979, and the South African Nature Foundation later bought adjoining land to reach today's size.

The park's name comes from the Karoo, the semi-desert region it protects. Springbok, the park's emblem, still gather in herds that recall the vast migrating treks that once crossed these plains. A quagga breeding programme uses Burchell's zebras to back-breed animals resembling the quagga, hunted to extinction in the mid-1800s; the last one died in Amsterdam Zoo on 12 August 1883.

Two main game-viewing drives need no 4x4: the eastern route stays on the Lammertjiesleegte plains, while the western 49 km loop climbs Klipspringer Pass to the plateau and returns via the Doornhoek picnic site. The middle section west of that loop is reached by 4x4. The camp has caravan and tent sites, chalets, a restaurant and a shop.

The area is home to five different tortoise species adapted to the harsh environment. This concentration of reptiles demonstrates how specialized wildlife can be in this ecosystem.