Anenské údolí, Nature reserve in Hroubovice and Skuteč, Czech Republic

Anenské údolí is a nature reserve and Natura 2000 site in the Chrudim District, with a narrow valley around the Anenský stream and the steep, south-facing Štěpánovská stráň escarpment. The protected area covers 74.19 hectares on two sites, at elevations from 320 to 420 metres, and includes Cretaceous sandstone and opuka beds.

The reserve was first declared on 1 February 2001 by the Chrudim District Office, covering 56.2 hectares. A 2014 regulation by the Pardubice Regional Office (No. 1/2014) enlarged it to 74.19 hectares across two sites, adding the Štěpánovská stráň slope between Zbožnov and Štěpánov.

The valley is named after Saint Anne, to whom the chapel built in 1831 above an iron-rich spring was dedicated; a legend links the saint to poor Marta of Štěpánov. By 1920 the site was a pilgrimage destination with tub and steam baths using the iron-lime water, and an excursion inn stood here.

The valley runs about 2 km from below Štěpánov (350 m) down to Hroubovice (320 m), with the chapel of St Anne roughly midway between Skuteč and Hroubovice. A local road follows the steep one-kilometre opuka slope of Štěpánovská stráň between Zbožnov and Štěpánov. At the upper edge of that slope, by the road, stands a self-service information point of the Železné hory National Geopark with rock samples from the reserve.

Scientists have documented nearly 100 vertebrate species within the reserve, making it an important site for monitoring biodiversity. This concentration of animal and plant life is noteworthy for a relatively small valley in this region.