Church of the Nativity of the Virgin Mary, Gothic church within Augustinian monastery in Roudnice nad Labem, Czech Republic.

The Church of the Nativity of the Virgin Mary is a Gothic structure featuring pointed arches, stone walls, and ribbed vaults characteristic of the era. Built as part of the monastery complex, it remains a focal point of the townscape and reflects regional building traditions.

Founded in 1333 by Prague bishop Jan IV of Dražice together with an Augustinian monastery, the church was consecrated to the Nativity of the Virgin Mary in 1340 and roughly completed by the 1360s, with work continuing until the Hussite wars. Damaged by the Hussite raid of 1421 and later fires, it passed to the Utraquists in 1467 and was rebuilt in Baroque style between 1725 and 1734.

The church was founded in 1333 by Prague bishop Jan IV of Dražice together with the Augustinian monastery, and the parish was attached to the monastery by a deed of 8 January 1334. From 1467 it served the Utraquists, and since 1 July 1994 the former provostship has been a parish again.

The church stands near the Labe at the corner of Komenského and Havlíčkova streets, a short walk from the town centre. It serves as the parish church, so visits are possible around services; the two west towers make it easy to find from the riverside.

The Romanesque church that stood on this site was not demolished: it was adapted and incorporated into the new Gothic building as the sacristy, so a visitor can still find the older structure inside the later church.