Chiesa e Monastero di Sant'Antonio in Polesine, Benedictine monastery and church in Ferrara, Italy.

Chiesa e Monastero di Sant'Antonio in Polesine is a Benedictine monastery with a church in Ferrara featuring three chapels adorned with painted frescoes. The interior displays works from the school of Giotto depicting scenes from the lives of Jesus and Mary.

The monastery was founded before 1000 on a Po River island and later became a center for Benedictine traditions and spiritual life. In 1257, Beatrice d'Este came to live here, bringing greater prominence to the place.

The name "in Polesine" refers not to the Polesine region but to the monastery's original setting on raised ground surrounded by water, an island in the Po. The nuns' church keeps frescoes of the school of Giotto: the Stories of the Infancy of Jesus and Life of the Virgin on the left, painted 1315–1320, and the Stories of the Passion on the right. The central chapel's 16th-century grotesque ceiling, with pagan images in a church, recalls the monastery's ties to the Este court.

Visits are available Monday through Saturday with guided tours arranged for small groups. It is best to inquire in advance about available times since access is limited.

The name "in Polesine" does not refer to the Polesine region between the Adige and Po; it recalls that the monastery stood on the island of Sant'Antonio, raised ground surrounded by water south of the Byzantine castrum. The complex is dedicated to Anthony the Abbot, considered the founder of Christian monasticism.