Museo Nicolaiano, Art museum in old town Bari, Italy.

The Museo Nicolaiano occupies a 13th-century building behind the Basilica di San Nicola, identified as the Ospizio dei Pellegrini, next to the arch marking the Cittadella Nicolaiana. Its displays run over three levels: ground-floor rooms on the basilica's antiquity, Byzantine and Romanesque phases, a first floor with ex-votos and contemporary works, and an upper floor holding the basilica's treasure.

The museum opened on 6 February 2010 in a 13th-century building behind the Basilica, identified as the Ospizio dei Pellegrini, which had housed the Accademia Nicolaiana until 1979 and earlier the Basilica's archive. The display was renewed in 2019 to cover the saint's life. The ground floor traces the Basilica's phases from antiquity through the Byzantine and Romanesque periods with artefacts, documents and epigraphs.

The museum keeps the Basilica's treasure, gathered from gifts brought by guests who came to visit Saint Nicholas over the centuries. On the top floor, objects are shown to trace how the saint's image and life evolved and his bond with Bari. The first floor holds ex-votos, some donated by the Orthodox community, and contemporary artworks.

The museum sits in the old town and is easy to reach on foot when visiting the Basilica nearby. Plan enough time to explore all three rooms at a comfortable pace.

The collection holds illuminated manuscripts from the Basilica archives decorated with detailed illustrations and ornaments. These rare documents offer insights into how the city's religious and administrative life was organized during the Middle Ages.