Nostra Signora dell'Acquasanta Sanctuary, Baroque sanctuary in Acquasanta, Genoa, Italy.

The sanctuary of Nostra Signora dell'Acquasanta is a baroque church with a Latin-cross plan and a single nave, built between 1683 and 1718. Its facade, designed by Maurizio Reggio, and the two bell towers were completed between 1900 and 1911. Inside, Giuseppe Canepa frescoed the interior in the 18th century, and Rodolfo Gambini reworked it in 1911.

The sanctuary was built between 1683 and 1718, probably to a design by the Lombard architect Carlo Muttone, under the Pieve di Palmaro. Its main altar was begun in 1730 by Francesco Maria Schiaffino and finished by his pupil Carlo Cacciatori; the facade by Maurizio Reggio and the two bell towers were completed between 1900 and 1911. Two plaques inside recall the marriage of King Ferdinand II of the Two Sicilies to Maria Cristina of Savoy on 21 November 1832.

The name Acquasanta refers to the spring around which the hamlet grew, linked to a Marian apparition said to have occurred in the mid-15th century. Each Sunday between August and September, confraternities from Mele, Arenzano, Crevari, Voltri and Pra' take turns processing here with their Christs, climbing the Scala Santa; at the top the bearers raise and lower the processional cases rhythmically in greeting to the Madonna.

The sanctuary stands in Piazza Chiesa dell'Acquasanta in the Acquasanta frazione of Genoa. The Scala Santa, a 33-step stair built from the Cappellina toward the sanctuary, is a separate devotional route, not the church entrance. Between August and September, confraternities from Mele, Arenzano, Crevari, Voltri and Pra' take turns arriving on Sundays.

The Scala Santa has thirty-three steps, a copy of the stairway said to have been climbed by Jesus Christ before Pilate. It leads from the Cappellina toward the sanctuary, and each Sunday between August and September one of the confraternities of Mele, Arenzano, Crevari, Voltri and Pra' climbs it in procession with its processional Christ, which the bearers raise and lower rhythmically at the top as a greeting to the Madonna.