Kumamoto City Zoological and Botanical Gardens, Zoological and botanical gardens in Higashi-ku, Kumamoto, Japan.

Kumamoto City Zoological and Botanical Gardens combines animal exhibits and plant collections across several zones, including enclosures, greenhouses, and flower gardens. The grounds also feature educational spaces and recreational areas designed for various interests.

The zoo opened in 1929 beside Suizenji Park with 9,900 m² and some animals transferred from Haneda Villa in Hiroshima. In 1944, 15 large animals of nine species were killed under wartime policy; elephant Ellie was electrocuted in 1945. It moved to the Lake Ezu shore in 1969 as a 102,000 m² 'waterside zoo', and in 1991 took its present name after the Greenpic '86 plant zone became a botanical garden.

The name changed from Kumamoto Zoo to Kumamoto City Zoological and Botanical Gardens in 1991, after the plant zone from Green Pick '86 was added. A statue of Tony Tony Chopper from One Piece was unveiled at the main entrance on 7 November 2020, the fourth such character statue in Kumamoto Prefecture. The keepers publish a monthly newsletter about the animals.

The grounds are split into an animal zone and a plant zone, linked by a south gate that leads into Suizenji-Ezu Lake Park. Trams and buses stop roughly 200 to 950 m from the entrances, with the west gate closest to the plant zone. Parking is free on weekdays and charged on weekends and holidays.

The zoo's former elephant, Ellie, was electrocuted in 1945 when the army ordered dangerous animals killed; her story inspired the choral suite "Sorry, Mary." A video of Ellie alive at the zoo surfaced in 2025. In 2020, a statue of Tony Tony Chopper from One Piece was installed at the main entrance.