Old Brno Abbey Museum, Abbey museum in Brno, Czech Republic.

The Old Brno Abbey Museum opened on 1 February 2018 inside the still-functioning Augustinian Abbey in Old Brno. Its four-part exhibition fills authentic monastic rooms, including the former dormitory of the Cistercian nuns and the Augustinians' assembly hall, and adds galleries of secular and sacred art.

The Cistercian convent was founded in 1323 by Queen Elizabeth Richeza under the name Aula Sanctae Mariae, with Christina as first abbess and twelve nuns installed before the charter of 1 June 1323. It was dissolved in 1782 by decree of Emperor Joseph II, and the Augustinians took over the vacant buildings the following year.

The museum's galleries occupy rooms of the working Augustinian abbey, including the former dormitory of the Cistercian sisters and the aula. The oldest paintings date from the 15th–16th centuries, and a double-sided panel shows the Nativity on one face and the Mocking of Christ on the other. A carved wooden door that once led to the sisters' choir bears a figure of Bernard of Clairvaux.

The museum opened on 1 February 2018 inside the still-functioning Augustinian Abbey in Old Brno, south of the city centre. Its four thematic sections and two art galleries occupy authentic monastic rooms, including the former dormitory of the Cistercian sisters and the aule. Mendel Museum arranges guided tours of the exhibition.

The museum's secular art gallery occupies the former dormitory of the Cistercian nuns. Its centrepiece is the largest painting of Jerusalem in the world, painted in 1937 by Brno-born Jewish artist Ludwig Blum for the Biblical Museum the Augustinians ran in the old chapter house; it has hung in the dormitory since 2000.