Regional Museum Mikulov, Historical museum in Mikulov Castle, Czech Republic.

The Regional Museum Mikulov occupies the ground floor of Mikulov Castle, where it moved in 1959. Its displays trace the natural and human history of the Břeclav region, from archaeology to viniculture, and include the castle's own collections. The museum is a contributory organisation of the South Moravian Region.

The Mikulov Museum Society was founded in 1913 by gymnasium director Arthur Petak, mayor Alois Winter and seventeen local enthusiasts. After a first permanent exhibition in the town hall in 1922, the museum moved to the ground floor of the reconstructed Mikulov Castle in 1959, opening a new exhibition on 10 May 1959. Its first curator, Karel Jüttner, led the institution until 1947 and died later that year.

The museum's library holds around 11,000 volumes from the former Piarist school, reflecting centuries of regional education traditions. This collection shows how important knowledge and learning have been to the area.

The museum occupies the ground floor of Mikulov Castle, where a new exhibition on the history of the Mikulov region opened on 10 May 1959. The branch in Dolní Věstonice closed its archaeological display after the Pavlov Archeopark opened in 2016; since 14 June 2024 the Baroque building there houses the Pálava House of Nature. The Horní Synagogue, owned by the Brno Jewish Community, is also managed by the museum.

In the castle cellar stands a massive wine barrel from 1643 that holds over 1,000 hectoliters and was once used to collect wine taxes. This unusual object is a rare surviving remnant from when wine was a major local resource.