Dr. William Rainey Holt House, Greek Revival residence in Lexington, United States.

The Dr. William Rainey Holt House, known as The Homestead, is a two-story, three-bay Greek Revival frame dwelling at 408 South Main Street in Lexington, North Carolina. It has a hipped roof, Palladian windows, and a double-pile center-hall plan. A rear addition dates to 1949, and a contributing former servants quarters called "Lizzie's house" stands on the property.

The house was built in 1834 for Dr. William Rainey Holt, a Pennsylvania-trained physician who practiced in Davidson County. During the Civil War, three of his sons were killed serving the Confederacy, and Union Army soldiers occupied his home. It was remodeled around 1892 and 1900, a rear addition followed in 1949, and it joined the National Register of Historic Places in 1983.

The Holt family gave the former servants' quarters on the property its own name, "Lizzie's house", and the building is counted as contributing to the site's historic character. The house's Palladian windows and sidelights follow details published in the pattern books of architect Asher Benjamin.

The house stands at 408 South Main Street in Lexington, Davidson County, with the former servants' quarters known as "Lizzie's house" on the same property. It is a private residence listed on the National Register of Historic Places since 1983, so visitors view the exterior from the street rather than touring the interior.

The Holt family called the surviving outbuilding on the grounds "Lizzie's house"; it served as servants' quarters and is a contributing structure to the National Register listing. The main house's Palladian windows and sidelights follow pattern-book designs by architect Asher Benjamin.