Stellenbosch University Botanical Garden, University botanical garden in central Stellenbosch, South Africa.

Stellenbosch University Botanical Garden sits between the university buildings in the centre of Stellenbosch and is the oldest university botanical garden in South Africa. It is small but holds indigenous and introduced plants, with two ferneries, two arid houses, water-lily ponds and glasshouses. The Western Cape Bonsai Heritage Collection and a collection covering about 70% of southern African Oxalis species are among its holdings.

The garden originated in 1902 when a lecturer began cultivating plants for research purposes, before the current site was established in 1922. Its development reflects the university's growing focus on botanical study over the decades.

The Western Cape Bonsai Heritage Collection presents South African bonsai traditions through one of the country's largest public collections. Visitors can see how this art form has taken root locally and continues to develop within the community.

The garden sits among the university buildings in the centre of Stellenbosch, with the Botanical Garden Shop serving as its visitor centre; the shop sells books, seeds and local plant products, and a small specialist nursery adjoins it. The Katjiepiering Restaurant is on the grounds. Stellenbosch's Mediterranean climate brings dry, warm summers and cool, rainy winters, with spring and autumn daytime temperatures around 20 °C.

The garden keeps Erica verticillata, a species extinct in the wild, and in 2018 was accredited as a Conservation Practitioner by Botanic Gardens Conservation International. Its Oxalis collection, begun in 2001, holds about 70% of southern African species and underpins molecular research on the genus.