Assmanshausen Winery, Heritage listed winery in Toolburra, Australia.

Assmanshausen Winery is a former winery at Serisier Road, Toolburra, in the Southern Downs Region of Queensland. Its dressed stone building has three levels: an underground cellar, a ground-level workroom and a steeply pitched shingled roof with an attic ventilated by 12 shuttered air shafts. Six sloped air shafts run through the cellar walls to the surface.

The winery was established in the 1860s by German immigrants Jacob and Elisabetha Kircher, named after a wine region on the Rhine River. The operation developed over subsequent decades into a significant wine production site in the local district.

The name comes from Assmannshausen, a wine district on the Rhine, chosen by the German immigrant Kirchers. By 1876 the property was the principal vineyard and winery of the Warwick district, with 10 acres under vines, and its wines had won prizes at the Warwick and Brisbane exhibitions.

The former winery stands on private rural land at Serisier Road, Toolburra, about 11 km northwest of Warwick; it is not open for tours or tastings. The stone winery building, with its underground cellar, ground-floor workroom and shingled attic, can be viewed from the road. Access is by car; the site is a heritage place, so entry onto the property requires the owner's permission.

Jacob Kircher created a horse-drawn weeding device during the 1870s and experimented with innovative methods for moving and fermenting wine. These early improvements demonstrate how winemakers were already seeking better production methods over 150 years ago.