Dupree Gardens, Botanical garden in Land o' Lakes, Florida.

Dupree Gardens was a 25-acre botanical garden opened to the public on December 1, 1940, 17 miles north of Tampa on Ehren Cutoff in what is now Land O' Lakes, Florida. A 1948 American Automobile Association publication described winding paths, meandering streams and waterfalls. The original stone ticket gate still stands at the edge of a suburban housing development.

The garden opened on December 1, 1940, and initially attracted tens of thousands of visitors annually. After World War II began, fuel and tire rationing led to a sharp drop in tourism.

Dupree Gardens was billed as the "Blossom Center of Florida" and drew over 30,000 visitors a year before wartime gas and tire rationing cut tourist traffic. A 1948 American Automobile Association guide called the 900-acre estate one of Florida's major attractions. The original stone ticket gate still stands on Ehren Cutoff, now at the edge of a suburban housing development.

The site was accessible via winding paths that led visitors through different levels, offering views across the varied terrain. An old stone entrance gate on Ehren Cutoff still marks the former access point at the edge of a residential development.

The stone ticket gate at the original entrance on Ehren Cutoff still stands, now at the edge of a suburban housing development. A historical marker there identifies the road that once led to the 25-acre garden.