Mission Santa Barbara, Spanish mission station in Santa Barbara County, United States

Mission Santa Barbara is a Spanish mission station in Santa Barbara County, California, positioned on a hill above the city. The complex consists of a church with two bell towers, an interior courtyard with a fountain, cloister buildings, and an expansive garden area with olive trees and palms.

Fermín Lasuén founded Mission Santa Barbara on December 4, 1786, as the tenth of the Alta California missions. Three earlier chapels, the last an adobe structure of 1793–94, were destroyed by the earthquake of December 21, 1812. The present church was begun in 1815 and largely finished by 1820, built in adobe by Chumash (Canalino) laborers, probably under master stonemason José Antonio Ramiez. The 1925 earthquake damaged the towers; they were rebuilt in 1927 and again between 1950 and 1953.

The church carries the title "Queen of the Missions" and displays a neoclassical facade that imitates Roman temples, bringing a European architectural style to early California. The cemetery holds graves of Chumash congregation members alongside early Spanish settlers, reflecting the encounters and tensions of that era.

A museum next to the church displays objects from the mission period and explains daily life during Spanish colonial times. The gardens and cemetery can be visited freely, while entry to the church interior and museum typically requires an admission contribution.

An underground aqueduct system from the late 18th century supplied the mission with water from a nearby creek and still partly functions today. The stone fountain in the courtyard was built by Chumash workers under the guidance of Spanish master masons and shows a blend of indigenous and European craft techniques.