San Giovanni in Ayno, Former church in Via di Monserrato, Rome, Italy

San Giovanni in Ayno is a former church at the corner of Via di Monserrato and Piazza de' Ricci, its tall narrow facade of orange-brown brick framed by pale stone pilasters and topped by a stone cornice. A long stone frieze across the upper facade carries the letters "AN SAL CHRISTIANAE M DDC". The dark wooden double door sits in a stone frame beneath a semicircular fanlight, flanked by grilled rectangular windows and blind arched panels of pale plaster.

The building first appeared in papal documents in 1186 as an affiliate of San Lorenzo in Damaso and operated as a parish church until 1824. For more than 600 years it served as a center of worship and community life in this quarter.

The church was the seat of the Confraternita dell'Orazione e Morte from 1552 to 1571, and the brotherhood had the building restored. Its titular saint was John the Evangelist, not John the Baptist, a point that shaped the debate over the name Ayno. The two side altars, dedicated to John the Evangelist and to Saint Anne, were built in the last two decades of the 17th century.

The facade faces the corner of Via di Monserrato and Piazza de' Ricci, so it is seen on foot while walking through the Regola quarter. The building is privately owned and not open to visitors; the brick front, the stone portal and the inscribed frieze are viewed from the street. The interior was divided by a floor in 1913 and is not accessible.

The 1728 canvas of Saint John the Evangelist signed "J.C." was not found in the building but in a private collection in poor condition, identified at the end of 2013. It is thought Giovanni Conca painted it for the church's reconsecration on 30 November 1729. The work, recorded by Nibby and other authors, had dropped out of accounts after the church was stripped of its artworks by 1911.