Santo Spirito dei Napoletani, Catholic church on Via Giulia in Regola district, Rome, Italy

Santo Spirito dei Napoletani is a single-nave baroque church on Via Giulia in the Regola district, with side chapels and a facade rebuilt in 1853 by Antonio Cipolla in neo-Renaissance style, featuring a sculpted portal, rose window and Pietro Gagliardi's fresco of the Holy Spirit in Glory. Inside, the left chapels hold Luca Giordano's Martirio di San Gennaro (1705) and an early 16th-century fresco of the Virgin and Child attributed to Antoniazzo Romano's workshop.

The church was first recorded as S. Aura in strada Iulia, dedicated to Saint Aurea of Ostia, and in the 14th century was also called Sant'Eusterio. In 1572 the dilapidated church and monastery were assigned to the Confraternity of the Holy Spirit of the Neapolitans, who demolished them and built a new church inaugurated in 1574. It was rebuilt at the end of the 16th century, redesigned by Carlo Fontana in the early 18th century, and restored by Antonio Cipolla in 1853. From 1934 to 1984 it held the tombs of Francis II of the Two Sicilies, Queen Maria Sophie and their daughter Cristina, transferred to Santa Chiara in Naples in 1984.

This church served as a gathering place for Neapolitans living in Rome and reflects their ties to their homeland through its art and design. Visitors can observe how the community maintained its identity and faith within these walls over the centuries.

The church is located at Via Giulia 34, within walking distance of other sights in the historic center. Visitors can enter during daytime hours, though opening times may be limited on certain days.

The church kept the remains of Francis II, Queen Maria Sophie and their daughter Cristina Pia, who died at three months and had rested there since 1870. In 1984 a solemn ceremony moved the three bodies to Santa Chiara in Naples.