Hospital de la Almudena, Colonial hospital in Santiago District, Cusco, Peru.

Hospital de la Almudena is a colonial building in Cusco's historic center with thick stone walls, vaulted corridors, and an interior courtyard typical of that era. The complex contains interconnected rooms that reveal how medical functions were organized during Spanish rule.

The hospital was founded during the colonial period to serve both Spanish settlers and the indigenous population with medical care. Its structure and development reflect the medical needs and challenges of Cusco under Spanish rule.

The hospital's name comes from the Plazoleta de la Almudena where it stands, beside the Templo de la Almudena and the Cementerio General. Founded on 29 June 1689 by fray Rodrigo de la Cruz, who had arrived in Cusco with ten religious of the Bethlehemite order, it served priests and sick indigenous people until the 20th century.

The building stands on the Plazoleta de la Almudena, beside the Templo de la Almudena, the Cementerio General de La Almudena and the Hospital de Salud Mental San Juan Pablo II, in the Santiago district rather than on the central plaza. It now houses offices of the Sociedad de Beneficencia Pública del Cusco, so visitors see the exterior and the small square; the site lies within the UNESCO-listed historic centre of Cusco, declared in 1983.

The building now houses offices of the Sociedad de Beneficencia Pública del Cusco, not a museum collection. It stands on the Plazoleta de la Almudena beside the Templo de la Almudena, the Cementerio General and the San Juan Pablo II mental health hospital. Since 1972 it has formed part of Cusco's Monumental Zone.