Santuário Nacional de São José de Anchieta, Colonial church in Anchieta, Brazil.

The Santuário Nacional de São José de Anchieta consists of the Church of Our Lady of the Assumption and the remaining wings of the old Jesuit residence, on a hillside above the Benevente River in Anchieta, Espírito Santo. The church was built in stone and lime in 1579; the residence later served as town hall, jail and courthouse, and its inner courtyard was used as the town cemetery in the 19th century.

The church was built in the late 16th century when the Jesuit priest José de Anchieta was active in this coastal region, teaching reading and Catholic faith to local communities. After his death, the site grew into a pilgrimage center and was eventually elevated to the status of a national sanctuary.

The name Reritiba, the village where the complex stands, survives in local use; the town later became Vila de Benevente in 1759 and is now Anchieta. The National Museum of Saint Joseph of Anchieta, created on the site in 1965, keeps old liturgical objects from the church and objects from the former village, which visitors can see alongside the church and residence.

The complex sits on a hillside above the Benevente River, reached by a slope from the town centre of Anchieta. The church and the former Jesuit residence stand side by side, with the São José de Anchieta National Museum housed in the residence since 1965. The inner courtyard and parts of the south and west wings once served as the town cemetery.

José de Anchieta, in whose honor the sanctuary was built, spoke several indigenous languages and wrote religious texts in Tupi, the most widely spoken language in the region at the time. These texts are considered among the earliest written records in an indigenous Brazilian language.