Matriz de Nossa Senhora da Imaculada Conceição, Catholic church in Matias Cardoso, Brazil.

The Matriz de Nossa Senhora da Imaculada Conceição in Matias Cardoso measures 33 m long and 20 m wide, with two 20 m towers. Its load-bearing walls are of burnt brick, giving it a fortress-like outline, and a low wall with columns at the corners and gates surrounds it. Along the sides, semicircular arches form a ground-floor veranda beneath the upper corridors.

Construction took place between 1670 and 1673 during the bandeirantes expeditions, making it the oldest religious structure in Minas Gerais. Its establishment during this period of exploration and settlement established its role as a key center in the region's early development.

The church is dedicated to Nossa Senhora da Imaculada Conceição, a devotion brought by the paulista settlers who founded the Morrinhos settlement. Its altar once held 17th-century Portuguese polychrome wooden images, among them Sant'Anna Mestra, Nossa Senhora de Bonsucesso and São Miguel, stolen on 13 August 2008 in a case pursued by the Federal Police and Interpol.

The church stands at Praça Cônego Maurício, about 300 meters from the São Francisco River bank. It is an active place of worship and accessible to visitors interested in religious architecture and the region's history.

The church was listed by IPHAN in 1954 after a technical opinion written by Carlos Drummond de Andrade, then head of the history section of SPHAN, and endorsed by architect Lúcio Costa. In 2008, 17th-century Portuguese polychrome wooden images of Sant'Anna Mestra, Nossa Senhora de Bonsucesso and São Miguel were stolen from the altar; the Federal Police and Interpol took part in the recovery effort.