Polk Museum of Art, Art museum in Lakeland, Florida.

The Polk Museum of Art is an art museum in Lakeland that displays American and European works across nine galleries. The collection includes paintings, sculptures, and decorative objects from different time periods and artistic styles.

The Junior Welfare League founded it in 1966 as the Imperial Youth Museum. Renamed Polk Public Museum in 1969, it broadened into art, history and science, and in 1970 its trustees bought a vacant Publix supermarket, doubling exhibition and classroom space. The Polk Museum of Art name came with the 1980s building campaign; architect Ernie Straughn's building opened in 1988. It affiliated with Florida Southern College on 1 June 2017 and was renamed the Ashley Gibson Barnett Museum of Art in 2024 after a donation from the Nicholas and Ashley Barnett Foundation.

The museum's name changed in 2024 to the Ashley Gibson Barnett Museum of Art after a donation from the Nicholas and Ashley Barnett Foundation; Nicholas is a grandson of Publix founder George W. Jenkins. Its African art collection began in 2004 with a gift of 56 traditional pieces. A virtual exhibit, "Hindsight 2020," shows works made between March and August 2020.

The museum is located on East Palmetto Street and offers free admission to all visitors. It stays open daily except for Mondays and national holidays.

The museum's building was designed by architect Ernie Straughn and completed in 1988, the same period when the Polk Museum of Art name was adopted during its first building campaign. The board had acquired a vacant Publix supermarket in 1970, doubling exhibition and classroom space.