Chiesa di Santa Margherita in Trastevere, Renaissance parish church in Trastevere, Rome, Italy.

Chiesa di Santa Margherita in Trastevere stands on Piazza di Sant'Apollonia in Rome's Trastevere district. Its two-tier facade is crowned by a cross with palm branches, and the front bears a double dedication to Saint Margaret, virgin and martyr, and Saint Emygdius, bishop and martyr. Inside, a single nave with a barrel vault and a semicircular apse opens onto three side chapels.

The church was commissioned in 1680 by Cardinal Girolamo Gastaldi and rebuilt by architect Carlo Fontana, altering the original orientation that dated back to 1288. This transformation reflected the renewal movements of the Baroque period that reshaped many structures throughout Rome.

The high altarpiece is Giacinto Brandi's "Saint Margaret Defeats the Demon", painted 1680-83 for the rebuilt church; the oval canvases on its sides are by Giuseppe Ghezzi. In the left chapel hangs Baciccio's "Immaculate Conception between Saint Francis and Saint Clare". The facade carries a double dedication, to Saint Margaret and to Saint Emygdius, bishop and martyr.

The church stands at Piazza di Sant'Apollonia 13 in Trastevere, a short walk from Viale di Trastevere. The convent beside it has been converted into private flats, so the entrance is the only access. The Confraternita di Sant'Emidio officiated here until 1996; since 2 February 1997 a priest of the Vicariato di Roma has been entrusted with the church.

The church kept the name Santa Margherita della Scala and was also called Santa Elisabetta; its nuns lived as bizzoche, without enclosure vows, as acts of a visitation under Alexander VII record. The facade carries a double dedication, to Saint Margaret and to Saint Emygdius, bishop and martyr.