Edificio de la Aduana de Valparaíso, Historic customs building in Plaza Wheelwright, Valparaíso, Chile.

The Edificio de la Aduana de Valparaíso stands at the foot of Cerro Artillería, facing Plaza Wheelwright, on land reclaimed from the sea by the port's first seawall. It has two floors, a compact symmetrical plan and facades, and walls of rendered brick 1.20 metres thick on stone foundations. Its architect, John Brown Diffin, gave it a local version of North American post-colonial architecture.

The engineer Augusto Charme drew up the plans in 1844 for a site created by the port's first seawall. Work stopped for two years and resumed in 1846 under the American architect John Brown Diffin, who redrew the plans. The building opened on 1 September 1855 and survived the 1906 earthquake; it was declared a National Historic Monument in 1976.

During Chile's Cultural Heritage Day, the building is among Valparaíso's most visited, drawing 1,500 people on Sunday 29 May 2016. Customs staff and their families form the folk group Alcabala, which performs period dances, costumes and music there, a tradition for the date.

The building stands at the foot of Cerro Artillería, facing Plaza Wheelwright, and remains the working seat of the Regional Customs Directorate, so it is normally closed to visitors. Access is possible during the Día del Patrimonio Cultural, when guided tours are led by customs staff; on 29 May 2016 about 1,500 people came through.

Inside, the building preserves the original director's desk and an 1864 security vault that speak to its long operational history. The ceiling structures are made from Chilean larch wood, a building material rarely seen in contemporary construction.