The Bosque Mbaracayú Biosphere Reserve, Biosphere reserve in Canindeyú Department, Paraguay.

The Bosque Mbaracayú is a large protected area with subtropical forests, grasslands, and wetlands in the Jejuí River basin. The landscape consists of different forest types ranging from wet valleys to higher elevations.

The reserve was established in 1991 through cooperation between Paraguay's government, the Moisés Bertoni Foundation, the United Nations, and The Nature Conservancy. This international partnership helped secure the forests for the future.

Two indigenous groups live in the reserve's area of influence: the Aché, hunter-gatherers native to this forest who established contact with Paraguayan society relatively recently, and Guaraní communities, who now depend more on farming and wage labour than on hunting. The reserve is managed by the Fundación Moisés Bertoni, an NGO founded in 1988.

Visitors can explore the area through designated trails and observe wildlife throughout the year. It is advisable to use a local guide to learn more about the different habitats.

The area holds one of Paraguay's last large sections of Atlantic rainforest and shelters rare bird and mammal species. Many visitors are surprised by how many different animals live in this relatively unknown forest area.