Middleburg United Methodist Church, Protestant church building in Middleburg, Florida, United States.

Middleburg United Methodist Church is a wooden church at 3925 Main Street in Middleburg, Florida. Its exterior is covered in wide clapboards, and the interior uses wide random-width tongue-and-groove boards. The pews are hand-finished mahogany, and the bell in the steeple was cast in 1852.

The building was originally known as Methodist Episcopal Church at Black Creek before being listed on the National Register of Historic Places in March 1990. This designation acknowledged its importance to the religious heritage of Northeast Florida.

The church's name comes from Black Creek, where Methodist families met in each other's homes from 1823, with a circuit-riding pastor visiting by horseback about once a month. Today the congregation runs a food ministry serving more than 300 families a week, a clothes ministry, and opens its space to community groups and a local Spanish church.

The church stands at 3925 Main Street in Middleburg. The historic church holds an early service at 8:30, so visitors can attend in the original building. The site includes later additions: the Budington Building, the Sanctuary and the Family Life Center.

The bell in the steeple was cast in 1852 and bought in New York by George Branning, who also donated the lumber and labor for the church. Its first official tolling marked the death of Branning's son. Handmade nails from a local blacksmith shop remain visible in the mahogany pews.