Tsambika Monastery, Eastern Orthodox monastery on Mount Tsambika, Rhodes, Greece.

Tsambika Monastery is an Orthodox religious site on Rhodes consisting of two separate structures: the original complex perched on a hilltop and a newer facility located beside the main road below. The upper structure sits at an elevated position overlooking the surrounding landscape, while the lower facility provides easier access for those unable to make the climb.

A sanctuary of Artemis stood on this hilltop in Classical times, attested by an inscribed sacred law listing sacrifices and votive offerings to the goddess. The church was consecrated in 1760, as an inscription records, and renovated in 1770 under the monk Hatzigerasimos. Its carved iconostasis holds 19th-century icons, one signed by Michael Symaios in 1885.

The monastery celebrates the Nativity of the Virgin on 8 September, when pilgrims come from all parts of Rhodes. According to the legend, women hoping for a child should walk barefoot from Kato Tsambika up to the hilltop chapel and pray there. Children born after such a pilgrimage are named Tsambika or Tsambikos, names found in Greece only in families from Rhodes.

The uphill chapel, Kyra Tsambika, stands about 330 m above the sea, reached by a stone stairway that zigzags up the rocky slope from the parking area; the lower monastery, Kato Tsambika, lies about 1 km further on at roughly 100 m, right beside National Road 95. Wear firm footwear for the climb and carry water, as the steps are exposed. Both sites are busiest around 8 September.

A miraculous icon of the Virgin Mary is connected to this site; according to local tradition, it vanished from Cyprus and reappeared here multiple times, prompting the monastery's founding. This story of the traveling image draws pilgrims who view it as a sign of divine presence.