Orto Botanico dell'Università di Perugia, Botanical garden at University of Perugia, Italy.

The Orto Botanico dell'Università di Perugia is a 26,000 m² garden between Via San Costanzo and Via Romana, established in 1962 by Mario Bolli. It holds about 1,200 taxa arranged mainly by phylogenetic criteria, with a 700 m² greenhouse, aquatic plants, an arboretum, succulents, and a hydrangea collection.

Perugia's first botanical collection dates to 1768, but the current garden was established in 1962. It represents a continuous tradition of scientific plant collection spanning more than two centuries.

The medieval garden occupies the site of the Benedictine monks' former fishpond, between the walls of the Basilica of San Pietro. Its model is the Hortus conclusus, the walled garden of monastic life. The visit follows three parts: a symbolic reconstruction of the Garden of Eden, a sacred wood, and the beds of medicinal (Hortus sanitatis) and food plants (Hortus holerorum).

The garden is located in the Borgo XX Giugno area and is open daily for visitors to explore. Guided tours can be arranged upon request to learn more about the collections.

A large greenhouse houses rare plant species and specialized collections, including an extensive Hydrangea collection. These sections allow visitors to see unusual plants that would not naturally grow in the region.