Trinity Church in Nikitniki, Eastern Orthodox church in Tverskoy District, Moscow, Russia

The Trinity Church in Nikitniki is a red-brick church with white stone trim, built on a high podklet. Its bell tower rises in red-and-white bands to a green onion dome, while the main block carries five green onion domes above tiers of white arched surrounds and circular medallions. A lower red-and-white building with arched openings and a small tented roof stands at the front right.

A wooden church of Saint Nikita stood here until it burned down in the 1620s. The Yaroslavl merchant Grigory Nikitnikov commissioned the stone Trinity Church, with sources citing building work in 1631–1634 and 1653; scholars debate which date marks the surviving structure. In 1920 the church was closed and in 1934 transferred to the State Historical Museum.

The interior frescoes depict biblical scenes based on Dutch engravings, painted by artists from the Kremlin Armory in the 17th century. These works reflect how Moscow merchants connected Russian religious art with European influences during that era.

The church was returned to the Russian Orthodox Church in 1991, and services are now held in the lower podklet; the museum that occupied it was closed. The main upper church, with its 1652–53 frescoes, is not part of the regular parish tour. The building stands in Kitay-gorod, enclosed by a fence and hemmed in by modern office blocks.

Simon Ushakov created the icon of the Tree of Russian Rulers specifically for this church, and it now resides in the Tretyakov Gallery collection. This work demonstrates how the building held importance for depicting Russia's rulers through religious art.