Healesville Sanctuary, Wildlife park in Healesville, Victoria, Australia

Healesville Sanctuary, formally the Sir Colin MacKenzie Sanctuary, is a zoo for Australian native animals at Healesville in the Yarra Ranges of Victoria. It occupies 32 hectares (78 acres), formerly part of the Coranderrk Aboriginal reserve, where paths wind through bushland habitat areas for wallabies, wombats, dingoes, kangaroos and over 200 native bird species. The six-roomed weatherboard MacKenzie Cottage, built around 1920 by Jim Anderson and Tom Smith, is the only remnant of the site's early years.

Sir Colin MacKenzie set up the Institute of Anatomical Research in 1920 on 32 hectares formerly part of the Coranderrk Aboriginal reserve. The land passed to the Healesville Council around 1927, and the site officially opened as the Sir Colin MacKenzie Sanctuary on 30 May 1934. The first platypus bred in captivity was born there in 1943 under David Fleay, and the Victorian Zoological Parks and Gardens Board took over management on 27 June 1978.

The sanctuary occupies land that was formerly part of the Coranderrk Aboriginal reserve before it passed to the Healesville Council around 1927. The MacKenzie Cottage, built around 1920 by local builders Jim (Scotty) Anderson and Tom Smith as a caretaker's cottage, is the only remnant of the site's early years and was added to the Victorian Heritage Register on 7 January 1999.

The grounds open daily from 9 in the morning, and most animal presentations take place throughout the day. The park has cafes, shaded rest spots, and paths suitable for strollers and wheelchairs.

In 1943 this facility achieved the first successful breeding of a platypus in human care anywhere in the world, a breakthrough that helped scientists better understand the biology of this unusual species. The sanctuary has since maintained a specialized breeding program that provides insight into the reproduction and care of these shy animals.