Pinellas National Wildlife Refuge, Wildlife refuge in St. Petersburg, United States.

Pinellas National Wildlife Refuge is a 394-acre refuge of four islands — Indian, Tarpon, Mule and Jackass Keys — lying offshore from St. Petersburg and reachable only by boat. The low, flat keys sit within the city limits and hold nesting colonies of brown pelicans, herons, egrets and cormorants.

The refuge was established in 1951 to create breeding grounds for seabirds and protect their colonies from disturbance. This protection developed from the understanding that these bird species needed quiet and safe areas to successfully raise their young.

The refuge is closed to public use, so the islands are not visited; the brown pelicans, herons, egrets and cormorants that nest there do so without human presence. Tarpon Key holds the largest brown pelican rookery in Florida. The name Pinellas comes from the Spanish Punta Piñal, "point of pines".

The refuge is closed to all public use to protect its natural and cultural resources, so there are no trails, docks or viewing points on the islands. It lies offshore from mainland St. Petersburg and can only be reached by boat, and even then landing is not permitted. The four keys—Indian, Tarpon, Mule and Jackass—sit within St. Petersburg city limits.

Tarpon Key, one of the four keys in the refuge, holds the largest brown pelican rookery in Florida. The refuge's 394 acres lie offshore of mainland St. Petersburg and are reachable only by boat, so the colonies stay undisturbed.