Tchouprene Biosphere Reserve, Biosphere reserve in northwestern Bulgaria.

Tchouprene Biosphere Reserve covers 1,451.99 hectares on the ridge and northeastern slopes of the Chiprovtsi mountain in the western Stara Planina. Forests take up about 90% of the area, mostly spruce and fir, with spruce stands over a century old. The terrain rises from about 1,100 m in the northwest to Replyanska Tserkva peak at 2,004 m in the southeast.

The reserve was established by the Bulgarian government in 1973 and gained UNESCO recognition in 1977 as part of the international biosphere program. This designation made it an officially recognized site for observing and protecting natural systems.

The reserve attracts researchers and nature enthusiasts who come to observe and study the plants and animals found here. Over time, it has become an important place for understanding how Balkan forests and wetlands work together.

The area is most easily reached from nearby villages, with marked trails leading through different parts of the reserve. Visitors should bring weather-appropriate clothing and be aware that conditions vary greatly depending on the season.

Tchouprene is the only Bulgarian reserve permanently inhabited by wolves. It also protects the country's only natural capercaillie population, and 170 bird species have been recorded here, alongside 53 mammal species including 14 bat species.