Handschuhsheim Cemetery, Cemetery in Handschuhsheim district, Heidelberg, Germany.

Handschuhsheim Cemetery covers 6.5 hectares on Neugasse, making it the second largest of Heidelberg's 17 cemeteries. It was laid out in 1842 through communal labour and extended several times: eastward onto rising ground in 1872, four times between 1948 and 1962, and north to Steinbergweg in 1980. A 4,000-square-metre Jewish section was consecrated in September 2016.

Handschuhsheim Cemetery was consecrated on 26 March 1843 as the first municipal cemetery of Handschuhsheim, then an independent community (incorporated into Heidelberg in 1903). It replaced the churchyard around the Vituskirche, which had been shared by Reformed and Catholic residents and was overcrowded; complaints about graves being reused too quickly date to 1812. The new ground on the Neugasse was laid out in 1842 through communal labour, and the planned opening of 19 March 1843 was postponed a week after unknown persons destroyed a stone cross set up by the Catholic community.

The cemetery's name comes from the Handschuhsheim district, which was an independent municipality until 1903. A 1784 bell from the former Lutheran church at Obere Kirchgasse 20 hangs in the bell tower added to the mortuary in 1949. Since September 2016 a 4,000-square-metre Jewish section has been consecrated here.

The cemetery lies on Neugasse and was later extended north to Steinbergweg; the upper section added in 1872 climbs the rising ground to the east. A 4,000-square-metre Jewish section was consecrated in September 2016. Grave locations are given by section and plot number, such as Abt. E, Grab 451-452 for Walther Bothe.

The cemetery holds the graves of two Nobel laureates: physicist Walther Bothe (1891–1957, Dept. E, grave 451-452) and chemist Georg Wittig (1897–1987, Dept. B, grave 158). Also buried here are Walther and Alice Sommerlath, parents of Queen Silvia of Sweden. A 4,000-square-metre Jewish section was consecrated in September 2016.