Manju-ji, Rinzai Buddhist sub-temple in Higashiyama-ku, Japan

Manju-ji is a Rinzai sub-temple of Tofuku-ji in Higashiyama-ku, Kyoto, set apart from the main precinct after the 1935 tram lines cut through it. Its guest hall (hojo), a Kyoto Prefecture designated cultural property, serves as the main hall. At the entrance stands a Muromachi-period bell tower gate, an Important Cultural Property originally built for Sansho-ji, with a bronze bell hanging in its upper storey above the passageway.

Manju-ji began as Rokujo Mido, a hall Emperor Shirakawa built at Rokujo Palace to pray for his daughter, Princess Taishi (Yuhomon-in), who died in 1096 aged 21; her death also prompted his taking Buddhist vows. In 1257-1259 Jichikaku and his disciple Hozan Tanzo placed it under Enni of Tofuku-ji, making it a Rinzai temple renamed Manju-ji, with the opening ceremony in 1261. After a 1273 fire it moved in 1330 to land granted by Somon-in, daughter of retired Emperor Go-Uda.

The temple keeps a silk portrait of the Chinese monk Nanpo Shomyo (1235–1308), who was appointed abbot in 1305, and a set of esoteric paintings used to teach the life of the Buddha. Its 281.8 cm wooden Amida statue, moved to the Kyoto National Museum in the Meiji era, is now in Tofuku-ji's Komyo Hall, not at Manju-ji.

Manju-ji is about a three-minute walk from Tofukuji Station on the Keihan Main Line and the JR Nara Line. The temple is closed to the public, so the grounds and the guest hall cannot be entered. The bell tower gate at the entrance, an Important Cultural Property, can be seen from outside.

Between 1257 and 1259, the temple shifted its religious direction from the Tendai Pure Land sect to Rinzai Zen under the influence of Enni. This transformation marked a turning point in how the temple practiced Buddhism.