Shizushi Limestone Cave, Limestone cave in Kyotamba, Japan.

Shizushi Limestone Cave is a vertical cave in Shizushi, Kyotamba, Kyoto Prefecture, about 52.5 m long with a height difference of roughly 25 m. It lies in limestone between basalt lava at about 400 m elevation at the head of the Takaya River, forming a small karst landscape. Discovered in 1927, it is Kyoto Prefecture's only limestone cave.

The cave received designation as a natural monument of Kyoto Prefecture, making it a significant research location for regional geology. This protected status has enabled ongoing scientific study of its formations.

The cave is a designated breeding site for cave bats such as the Japanese horseshoe bat, and it lies in the Tanba Group strata where fossils of Early Permian fusulinids and conodonts have been found. The surrounding Shizushi Limestone Cave Park is used for camping, hiking and barbecues.

Access to the cave requires descending a series of metal stairs built into the rock. Good footwear is recommended, and visitors should prepare for cool underground temperatures.

The cave lies in limestone wedged between basalt lava at about 400 metres elevation, the top of the Takaya River's upper course, where a small karst landscape has formed. The surrounding Shizushi area belongs to the Tamba Group, and fossils of Early Permian fusulinids and conodonts have been found there.