Longleaf Trace, Rail trail in South Mississippi, United States.

The Longleaf Trace is a 44-mile (71 km) paved trail for walking, cycling, rollerblading and horseback riding between Hattiesburg and Prentiss, Mississippi. It follows part of the abandoned Mississippi Central Railroad line and has nine stations along its route, from Prentiss to Jackson Road.

The route follows a railroad built in the 1800s that once supported the timber industry through the early 1900s. In 2000 the abandoned rail line was converted into a public recreation trail.

The trail's name comes from the longleaf pine, the tree that fed the sawmills served by the Mississippi Central Railroad line it now follows. Nine named stations mark the route, from Prentiss through Bassfield and Sumrall to Jackson Road, and between Hattiesburg and Sumrall many trees carry identification signposts for walkers.

Access is only from public-road points, spaced about 5 miles apart. In Hattiesburg, rest stops and parking lots serve the trail at the University of Southern Mississippi, West 4th Street and Jackson Road. Rest stops appear roughly every two miles, and mile markers aid navigation. The steepest segment rises 50 feet per mile.

The trail has nine stations along its route, from Prentiss through Carson, Bassfield, Lott Circle, Sumrall, Epley and Clyde Depot to Jackson Road. Many trees between Hattiesburg and Sumrall carry identification signposts, so walkers can name the flowering dogwood, magnolia, honeysuckle and wisteria they pass.