John's Island Presbyterian Church, Historic Presbyterian church on Johns Island, South Carolina.

John's Island Presbyterian Church is a wooden T-shaped house of worship with two entrance doors at the front and arched windows framed with wooden shutters. The building occupies several acres of land and preserves its original interior arrangement with balcony seating.

John's Island Presbyterian Church was founded in 1719 by Rev. Archibald Stobo, a Church of Scotland minister. The frame meeting house was remodeled in 1792 and extended in 1823, giving it its T-shaped plan. It was listed on the National Register of Historic Places in 1975.

The building follows the simple design of colonial meeting houses, with a basic wood frame and interior balcony that shows how congregations worshipped in early American times. Visitors can still see how the space was arranged to bring people together for prayer and preaching.

The church stands on Johns Island, west of Charleston, and is reached by road from the city. Visitors enter through the two front doors of the T-shaped frame building; the clapboard exterior and arched windows with wooden shutters can be seen from the grounds. The building was added to the National Register of Historic Places in 1975.

This wooden church ranks among the oldest continuously operating ones in South Carolina, and it still functions much as it did centuries ago. That unbroken continuity makes it rare evidence of religious practice extending back to colonial times.