San Giovannino dei Cavalieri, Renaissance church in Via San Gallo, Florence, Italy

San Giovannino dei Cavalieri is a church on Via San Gallo in Florence with a stone facade from the 1600s. The interior combines classical architectural details with Renaissance design principles throughout its spaces.

The site began as an oratory dedicated to Mary Magdalene with a hospice for repentant women. Around 1326–1327 it passed to the Celestines, who were called 'del Murrone' after their founder Pietro da Morrone, and the church became San Pier del Murrone. In 1552 Cosimo I de' Medici moved the Knights of Malta nuns here from San Salvatore di Camaldoli; they rebuilt the church, reconsecrated on 9 April 1553, and the dedication to St John the Baptist prevailed. The convent was suppressed in 1808.

The name San Giovannino dei Cavalieri comes from the Knights of Malta, who took over the convent in 1552; their white cross on a red field, held by two marble angels, stands on the facade. The church became the chosen convent of Florentine nobility, and Maria Maddalena de' Pazzi entered here in 1580.

The entrance is on Via San Gallo, but the door opens into a vestibule rather than the nave: this anteroom, financed largely by Cosimo I, holds large cupboards built to store the order's paper archive, and marks in the wall show a wooden bench once ran around it. The church itself lies beyond, entered under a marble tympanum bearing a Medici coat of arms.

The Annunciation attributed to the Master of Stratonice is one of several old panels here, most of which came with the nuns' dowries; the church also keeps a painted cross in the apse by Lorenzo Monaco and a Last Supper by Palma il Giovane.