Santa Maria Immacolata a Villa Borghese, Religious edifice in Villa Borghese park, Rome, Italy

Santa Maria Immacolata a Villa Borghese is a Catholic church housed in the Casina di Raffaello, a former farmhouse for the prince's tenants and cultivators, on Piazza di Siena in the Pinciano district. Its characteristic feature is a portico with four Doric columns. Inside, frescoes by Felice Giani (1791–1793) and later work by Pietro Carrarini (1829) cover the walls.

The church occupies the Casina di Raffaello, a farmhouse for labourers and cultivators of the Borghese estate that Prince Marcantonio Borghese had converted at the end of the 18th century, entrusting the work to architect Mario Asprucci. Felice Giani painted its frescoes between 1791 and 1793. Restoration and completion works finished in 1829, when Pietro Carrarini added further frescoes. It is now an auxiliary place of worship of the parish of Santa Teresa d'Avila.

The church is an auxiliary place of worship of the parish of Santa Teresa d'Avila, so it is not a separate parish church. It occupies the Casina di Raffaello, a former farmhouse for labourers and cultivators on the Borghese estate, converted for the Madonna. The name recalls the dedication to the Immaculate Conception given by Prince Marcantonio Borghese.

The church stands in the Casina di Raffaello complex on Piazza di Siena, in the Pinciano district inside Villa Borghese park. It is a subsidiary place of worship of the parish of Santa Teresa d'Avila, so opening depends on parish use rather than park hours. Approach on foot through the park paths; the entrance is under the portico with four Doric columns.

The church occupies the Casina di Raffaello, a former farmhouse for labourers and cultivators on the Borghese estate, converted at the wish of Prince Marcantonio Borghese. Its frescoes were painted between 1791 and 1793 by Felice Giani, and the 1829 restoration added frescoes by Pietro Carrarini.