Church of the Holy Cross, Nicosia, Roman Catholic church in central Nicosia, Cyprus.

The Church of the Holy Cross is a Roman Catholic parish church at the Paphos Gate in Nicosia, run by the Franciscans of the Custody of the Holy Land. The present building was financed partly by the Spanish Royal Family and inaugurated on 16 February 1902. A friary beside it was rebuilt in 1959, and a marble tablet with a Latin inscription stands in the first arch.

The first Church of the Holy Cross was built in 1642 and used until the end of the nineteenth century. The present church was financed partly by the Spanish royal family and partly by the Franciscans of the Custody of the Holy Land, begun on 8 April 1900 and formally inaugurated on 16 February 1902. The adjoining friary was rebuilt in 1959.

The parish belongs to the Latin Patriarchate of Jerusalem and draws worshippers from fifty different nations, with Masses celebrated in eight languages. A marble tablet with a Latin inscription stands in the first arch of the church. The friary beside it is run by Franciscans.

The interior underwent complete restoration in 1998 with new marble installations and an Italian-designed stained glass window installed above the altar. The church is situated near the buffer zone between the two parts of the city.

The church garden is located within the buffer zone between the divided city and remains partially restricted due to the 1974 territorial division. This geographic situation makes it an unusual example of a place affected by the island's modern history.