Museo de Historia Natural de Valparaíso, Natural history museum in Valparaíso, Chile.

The Museo de Historia Natural de Valparaíso occupies the Palacio Lyon, a building declared a National Monument of Chile in 1979. Founded in 1878 by Eduardo de la Barra, it is Chile's second-oldest museum and the oldest outside Santiago. By 1984 its collection held 25,856 pieces, and it has been housed in the palace since 1988.

The museum was established in 1878 and was at that time the first regional institution of its kind in Chile and the second oldest nationwide. This early founding made it an important place for preserving and presenting knowledge about the region.

The museum's name reflects its origins as a regional natural history institution, and the collection focuses on taxidermied animals and preserved specimens that show the wildlife of the area. The exhibition gives visitors a sense of which species are native here and how the natural world of the Valparaíso region appears.

The museum occupies the Palacio Lyon, a building declared a National Monument in 1979, in the centre of Valparaíso near the port and the city's funiculars. It is managed by the Servicio Nacional del Patrimonio Cultural, so opening arrangements follow that service rather than a fixed daily pattern; confirm before setting out.

The museum's collection held 25,856 pieces by 1984, and it received 7,150 visitors in 1980. Its founding director was Eduardo de la Barra, who established it in 1878 at the Liceo de Hombres de Valparaíso. Since 1988 it has occupied the Palacio Lyon, declared a National Monument in 1979.