Santuario de Nuestra Señora de Guadalupe, Catholic temple in Guadalajara, Mexico.

The Santuario de Nuestra Señora de Guadalupe is a Catholic temple on Paseo Alcalde in Guadalajara, built of brown stone blocks from the Huentitán ravine. Its facade rises in three vertical sections alluding to the Holy Trinity, the wider central one crowned by a curved gable with a carved relief. Twin bell towers with arched openings and bells flank it, topped by small stone cupolas, and a cylindrical stone buttress projects to the right of the centre.

Construction began in 1777 under Bishop Antonio Alcalde y Barriga, who financed the sanctuary. The builders also created nearby houses to support poor residents in the northern area.

The name honours the Virgin of Guadalupe, to whom Bishop Antonio Alcalde was devoted, and the church holds the rank of archdiocesan sanctuary. The image venerated at the main altar is a painting of 1779 by José de Alcíbar, set in a carved and gilded wooden niche. The interior is neoclassical, with gilded wooden retables; the facade alone keeps three vertical sections alluding to the Holy Trinity.

The sanctuary is located along Paseo Alcalde and can be reached on foot. The floor features decorative mosaic tiles beneath which burial spaces are situated, so visitors should move respectfully through the interior.

The facade's three vertical sections allude to the Holy Trinity, a scheme found only on the exterior; the interior follows neoclassical lines. The stone came from the Huentitán ravine, the same quarry that supplied Bishop Alcalde's low-rent houses, the "cuadritas", for poor residents of the Barrio del Santuario.