Kofa National Wildlife Refuge, Nature reserve in La Paz County, Arizona, United States.

Kofa National Wildlife Refuge spreads across desert terrain featuring rhyolite mountains, small canyons, and Sonoran Desert plants across elevations up to about 4,900 feet (1,500 meters). The landscape shows open, rocky ground with sparse vegetation adapted to extreme heat and drought conditions.

The refuge received protected status through Executive Order 8039 in January 1939 to safeguard the desert bighorn sheep population in the region. This action was one of the early efforts to protect desert habitats and endangered wildlife in the United States.

The name Kofa is a contraction of King of Arizona, a gold mine active from 1897 to 1910. The refuge's California fan palms in Palm Canyon are the only native palm in Arizona, likely relics of a wetter geological period. The Palm Canyon Trail has been a National Recreation Trail since 2007.

Visitors need four-wheel-drive vehicles to navigate unpaved roads and should carry plenty of water, especially when exploring between October and April. Proper preparation and emergency supplies are important since the area is remote with no nearby services.

General George S. Patton used Kofa for desert military training exercises during World War II, and unexploded ordnance may still be encountered during cross-country hiking. The refuge also holds the Kofa Mountain barberry, Berberis harrisoniana, a rare plant endemic to southwestern Arizona.