St. James A. M. E. Church, Gothic Revival church in Florida, United States.

St. James A. M. E. Church is a red brick Gothic Revival church at the corner of East Ninth Street and South Cypress Avenue in Sanford, Florida. It was designed by local architect Prince W. Spears and replaced the congregation's original building in 1913.

Founded in 1867 by African Americans in Sanford, the congregation bought land at East Ninth Street and South Cypress Avenue, making it the city's oldest church founded by African Americans. The original building was replaced in 1913 by a red brick Gothic Revival church designed by local architect Prince W. Spears. It was added to the National Register of Historic Places on April 24, 1992.

Founded in 1867, St. James is the oldest church in Sanford established by African Americans, and its congregation has worshipped on the corner of East Ninth Street and South Cypress Avenue since that year. The name follows the African Methodist Episcopal Church, the denomination the congregation belongs to.

The church stands at the corner of East Ninth Street and South Cypress Avenue in Sanford, the site it has occupied since 1867. The present red brick building dates from 1913. Visitors should arrange any tour in advance with the congregation, since the building is an active place of worship rather than a monument open on fixed hours.

The congregation's first church stood on the same corner before the 1913 brick building replaced it, so the site has been in use since 1867. The 1989 guide entry came three years before the April 24, 1992 listing on the National Register of Historic Places.