Winnipeg Railway Museum, Railway museum in Union Station, Winnipeg, Canada.

The Winnipeg Railway Museum occupies tracks 1 and 2 within Union Station and displays locomotives, railcars, and technical equipment spanning different railway eras. The space shows the evolution of rail technology across more than a century.

The Midwestern Rail Association, a non-profit founded in 1975, has operated the museum on tracks 1 and 2 of Union Station. The museum closed at the end of 2021 for renovations, and in November 2022 the association signed a 25-year lease with Via Rail, conditional on fixing fire code issues. By May 2024 roof, window, skylight and lighting upgrades were done, with reopening hoped for November 2025.

The museum was run by volunteers of the Midwestern Rail Association, a non-profit founded in 1975, and was affiliated with the Canadian Museums Association, CHIN and the Virtual Museum of Canada. Its mission was to explore the railway history of Winnipeg and Manitoba, and in 2015 restoration began on Car 356, Winnipeg's last wooden streetcar.

The museum is located within the historic Union Station right in the downtown core and is easy to reach. It is currently closed for renovations until November 2025, so visitors should check its status before visiting.

The collection holds a Jordan spreader built in 1911, a track-maintenance machine used to shape ballast and clear snow from the line. It sits alongside displays on the Hudson Bay Railway to Churchill, a line built across northern Manitoba to reach a Hudson Bay port.